New Procedure for Fumonisins B1 and B2 in Corn and Corn Products by Ion Pair Chromatography with o-Phthaldialdehyde Postcolumn Derivatization and Fluorometric Detection

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• 作者：Makoto Miyahara ; Hiroshi Akiyama ; Masatake Toyoda ; and Yukio Saito
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：1996
• 出版时间：March 1996
• 年：1996
• 卷：44
• 期：3
• 页码：842 - 847
• 全文大小：204K
• 年卷期：v.44,no.3(March 1996)
• ISSN：1520-5118

文摘

Fumonisins are a group of naturally occurring mycotoxins produced byFusarium moniliforme. Ananalytical method for fumonisins B₁ and B₂ incorn and related foods has been developed that utilizesion pair chromatography with a postcolumn reaction technique.Samples were prepared and purifiedby tandem solid phase extraction columns (SAX and C₁₈).The fumonisins were derivatized witho-phthaldialdehyde andN-acetyl-L-cysteine in an in-line reaction coil.Ion pair liquid chromatographywith fluorescence detection is used for the separation and detection.Absolute recoveries offumonisins ranged from 54 to 110% at 0.04 and 0.08 g/g. Thedetection limit of this method is 20ng. This procedure is relatively simple and provides a screeningmethod for fumonisins in cornand related foods.Keywords: Determination; fumonisin; solid phase extraction; ion pairchromatography; o-phthaldialdehyde; postcolumn reaction; fluorometric