Global Changes in and Characterization of Specific Sites of Phosphorylation in Mouse and Human Histone H1 Isoforms upon CDK Inhibitor Treatment Using Mass Spectrometry
详细信息 查看全文
- 作者：Leesa J. Deterding ; Maureen K. Bunger ; Geoffrey C. Banks ; Kenneth B. Tomer ; Trevor K. Archer
- 刊名：Journal of Proteome Research
- 出版年：2008
- 出版时间：June 2008
- 年：2008
- 卷：7
- 期：6
- 页码：2368 - 2379
- 全文大小：2904K
- 年卷期：v.7,no.6(June 2008)
- ISSN：1535-3907
文摘
Global changes in the phosphorylation state of human H1 isoforms isolated from UL3 cells have been investigated using mass spectrometry. Relative changes in H1 phosphorylation between untreated cells and cells treated with dexamethasone or various CDK inhibitors were determined. The specific cyclin-dependent kinase consensus sites of phosphorylation on the histone H1 isoforms that show changes in phosphorylation were also investigated. Three sites of phosphorylation on histone H1.4 isoforms have been identified.