Effects of Tyramine and 4-Aminophenol on the Oscillating Peroxidase–Oxidase Reaction

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• 作者：Andrew G. McDonald ; Keith F. Tipton
• 刊名：Journal of Physical Chemistry B
• 出版年：2014
• 出版时间：January 9, 2014
• 年：2014
• 卷：118
• 期：1
• 页码：18-25
• 全文大小：401K
• ISSN：1520-5207

文摘

The peroxidase鈥搊xidase oscillator, a model of biological oscillations, is usually studied in conjunction with the effector molecule, 2,4-dichlorophenol. In this account, we present evidence of the effects of a naturally occurring phenol, tyramine, on the reaction, and also those of the structurally similar 4-aminophenol. Whereas 2,4-dichlorophenol gives rise to sustained oscillations at 40 渭M, it was discovered that tyramine promotes damped oscillations at a concentration of 120 渭M. Oxidation of NADH was completely inhibited by 4-aminophenol and ascorbate. In separate experiments, the peroxidase-catalyzed ring coupling of tyramine and 4-aminophenol was observed, which in the case of tyramine, may provide an explanation for the damping of oscillations.