Proteomic Analysis of Amniotic Membrane Prepared for Human Transplantation: Characterization of Proteins and Clinical Implications

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• 作者：Andrew Hopkinson ; Richard S. McIntosh ; Vijay Shanmuganathan ; Patrick J. Tighe ; Harminder S. Dua
• 刊名：Journal of Proteome Research
• 出版年：2006
• 出版时间：September 2006
• 年：2006
• 卷：5
• 期：9
• 页码：2226 - 2235
• 全文大小：474K
• 年卷期：v.5,no.9(September 2006)
• ISSN：1535-3907

文摘

Amniotic membrane is commonly exploited in several surgical procedures. Despite a freeze preservationperiod, it is reported to retain wound healing, anti-angiogenic, antiinflammatory and anti-scarringproperties; however, little is known about the active protein content. 2-DE analysis of transplant-readyamniotic membrane (TRAM) was performed. The effects of preservation and processing on amnionproteome were investigated, and the major proteins in the TRAM characterized using mass spectrometryand immunoblotting. This identified a spectrum of proteins including thrombospondin, mimecan, BIG-H3, and integrin alpha 6. Preservation compromises cellular viability resulting in selective elution ofsoluble cellular proteins, leaving behind extracellular matrix-associated and cell structural proteins. Anumber of key architectural proteins common to the architecture of the ocular surface weredemonstrated in AM, which are involved in homeostasis and wound healing. Handling proceduresalter the protein composition of amniotic membrane prepared for transplantation. Without standardization, there will be inter-membrane variation, which may compromise the desired therapeutic effect oftransplant ready amniotic membrane.