Small Molecule Induction of Human Umbilical Stem Cells into Myelin Basic Protein Positive Oligodendrocytes in a Defined Three-Dimensional Environment
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- 作者：Hedvika Davis ; Xiufang Guo ; Stephen Lambert ; Maria Stancescu ; James J. Hickman
- 刊名：ACS Chemical Neuroscience
- 出版年：2012
- 出版时间：January 18, 2012
- 年：2012
- 卷：3
- 期：1
- 页码：31-39
- 全文大小：430K
- 年卷期：v.3,no.1(January 18, 2012)
- ISSN：1948-7193
文摘
Umbilical cord stem cells would be a favorable alternative to embryonic stem cells for therapeutic applications. In this study, human multipotent progenitor cells (MLPCs) from umbilical cord were differentiated into oligodendrocytes by being exposed to a range of microenvironmental chemical and physical cues. Chemical cues were represented by a novel defined differentiation medium containing the neurotransmitter norepinephrine (NE). Under traditional two-dimensional conditions, the MLPCs differentiated into oligodendrocyte precursors but did not progress further. However, in a three-dimensional environment, the MLPCs differentiated into committed oligodendrocytes that expressed myelin basic protein. The apparent method of interaction of NE in stimulating the differentiation process was shown to occur through the adenergic pathway, while all prior differentiation methods have used other routes. This novel method of obtaining functional human oligodendrocytes from MLPCs would eliminate many of the difficulties associated with their differentiation from embryonic stem cells.