Comprehensive and Systematic Analysis of the Immunocompatibility of Polyelectrolyte Capsules

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• 作者：Mikhail V. Zyuzin ; Paula Díez ; Meir Goldsmith ; Susana Carregal-Romero ; Cristina Teodosio ; Joanna Rejman ; Neus Feliu ; Alberto EscuderoMaría Jesús Almendral ; Uwe Linne ; Dan Peer ; Manuel Fuentes ; Wolfgang J. Parak
• 刊名：Bioconjugate Chemistry
• 出版年：2017
• 出版时间：February 15, 2017
• 年：2017
• 卷：28
• 期：2
• 页码：556-564
• 全文大小：602K
• ISSN：1520-4812

文摘

The immunocompability of polyelectrolyte capsules synthesized by layer-by-layer deposition has been investigated. Capsules of different architecture and composed of either non-degradable or biodegradable polymers, with either positively or negatively charged outer surface, and with micrometer size, have been used, and the capsule uptake by different cell lines has been studied and quantified. Immunocompatibility studies were performed with peripheral blood mononuclear cells (PBMCs). Data demonstrate that incubation with capsules, at concentrations relevant for practical applications, did not result in a reduced viability of cells, as it did not show an increased apoptosis. Presence of capsules also did not result in an increased expression of TNF-α, as detected with antibody staining, as well as at mRNA level. It also did not result in increased expression of IL-6, as detected at mRNA level. These results indicate that the polyelectrolyte capsules used in this study are immunocompatible.