Polyhedral Oligomeric Silsesquioxane as a Cross-linker for Preparation of Inorganic−Organic Hybrid Monolithic Columns

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• 作者：Minghuo Wu ; Ren’ ; an Wu ; Ruibing Li ; Hongqiang Qin ; Jing Dong ; Zhenbin Zhang ; Hanfa Zou
• 刊名：Analytical Chemistry
• 出版年：2010
• 出版时间：July 1, 2010
• 年：2010
• 卷：82
• 期：13
• 页码：5447-5454
• 全文大小：226K
• 年卷期：v.82,no.13(July 1, 2010)
• ISSN：1520-6882

文摘

An inorganic−organic hybrid monolithic capillary column was synthesized via thermal free radical copolymerization within the confines of a capillary using a polyhedral oligomeric silsesquioxane (POSS) reagent as the inorganic−organic hybrid cross-linker and a synthesized long carbon chain quaternary ammonium methacrylate of N-(2-(methacryloyloxy)ethyl)-dimethyloctadecylammonium bromide (MDOAB) as the organic monomer. The preparation process was as simple as pure organic polymer-based monolithic columns instead of using POSS as the nanosized inorganic−organic hybrid blocks (cross-linker) of the monolithic matrix. The pore properties and permeability could be tuned by the composition of the polymerization mixture. The characterization and evaluation results indicated that the synthesized MDOA−POSS hybrid monolith possessed the merits of organic polymer-based monoliths and silica-based monoliths with good mechanical and pH (pH 1−11) stabilities, which may be attributed to the incorporation of the rigid nanosized silica core of POSS. Column efficiencies of 223000 and 50000 N/m were observed in capillary electro-driven chromatography (CEC) and μ-HPLC, respectively. Peptides and standard proteins were baseline separated by this hybrid column in CEC and μ-HPLC, respectively, as well. The separation of bovine serum albumin (BSA) tryptic digest was also attempted to show its potential application in proteome analysis.