Biocompatible ZnO/Au Nanocomposites for Ultrasensitive DNA Detection Using Resonance Raman Scattering
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- 作者：Yichun Liu ; Mingya Zhong ; Guiye Shan ; Yajun Li ; Baiqu Huang ; Guoliang Yang
- 刊名：Journal of Physical Chemistry B
- 出版年：2008
- 出版时间：May 22, 2008
- 年：2008
- 卷：112
- 期：20
- 页码：6484 - 6489
- 全文大小：1532K
- 年卷期：v.112,no.20(May 22, 2008)
- ISSN：1520-5207
文摘
A novel method for identifying DNA microarrays based on ZnO/Au nanocomposites functionalized with thiol-oligonucleotide as probes is descried here. DNA labeled with ZnO/Au nanocomposites has a strong Raman signal even without silver acting as a surface-enhanced Raman scattering promoter. X-ray photoelectron spectra confirmed the formation of a three-component sandwich assay, i.e., constituted DNA and ZnO/Au nanocomposites. The resonance multiple-phonon Raman signal of the ZnO/Au nanocomposites as a spectroscopic fingerprint is used to detect a target sequence of oligonucleotide. This method exhibits extraordinary sensitivity and the detection limit is at least 1 fM.