Stabilization of C60 Nanoparticles by Protein Adsorption and Its Implications for Toxicity Studies
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- 作者：Shigeru Deguchi ; Tomoko Yamazaki ; Sada-atsu Mukai ; Ron Usami ; Koki Horikoshi
- 刊名：Chemical Research in Toxicology
- 出版年：2007
- 出版时间：June 2007
- 年：2007
- 卷：20
- 期：6
- 页码：854 - 858
- 全文大小：100K
- 年卷期：v.20,no.6(June 2007)
- ISSN：1520-5010
文摘
Dispersion stability of nanoparticles of C60 under a model condition simulating a physiologicalenvironment was studied by dynamic light scattering. Although the C60 nanoparticles at a concentrationof 9.3 × 10-6 M (6.7 
g/mL) coagulated and precipitated out rapidly in phosphate buffered saline,coagulation was suppressed completely when HSA was present at concentrations above 1 mg/mL. DLSresults show that the HSA molecules adsorb onto the surfaces of the C60 nanoparticles, thereby forminga protective layer, and prevent salt-induced coagulation. DLS results also indicate that the HSA moleculestake an expanded conformation on the surface. Our findings suggest that C60 nanoparticles can be stabilizedin the physiological environment even if they are not deliberately stabilized by using stabilizers and areof significant implications for the on-going efforts to evaluate the cytotoxicity of C60 nanoparticles inwhich no such effect has been considered.
g/mL) coagulated and precipitated out rapidly in phosphate buffered saline,coagulation was suppressed completely when HSA was present at concentrations above 1 mg/mL. DLSresults show that the HSA molecules adsorb onto the surfaces of the C60 nanoparticles, thereby forminga protective layer, and prevent salt-induced coagulation. DLS results also indicate that the HSA moleculestake an expanded conformation on the surface. Our findings suggest that C60 nanoparticles can be stabilizedin the physiological environment even if they are not deliberately stabilized by using stabilizers and areof significant implications for the on-going efforts to evaluate the cytotoxicity of C60 nanoparticles inwhich no such effect has been considered.