Development and Characterizations of a Miniature Capillary Electrophoresis Mass Spectrometry System

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• 作者：Muyi He ; Zhenhua Xue ; Yinna Zhang ; Zejian Huang ; Xiang Fang ; Feng Qu ; Zheng Ouyang ; Wei Xu
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：February 17, 2015
• 年：2015
• 卷：87
• 期：4
• 页码：2236-2241
• 全文大小：386K
• ISSN：1520-6882

文摘

A miniature capillary electrophoresis mass spectrometry (CE/MS) system has been developed in this work. A 100% electrical driven miniaturized CE device was integrated with a miniature MS instrument, which has a discontinuous atmospheric pressure interface (DAPI) for coupling with atmospheric pressure ionization sources. A nanoelectrospray ionization (nano-ESI) source was developed with a sheath liquid interface for coupling the miniature CE and the MS system. A systematic characterization and optimization of the analytical performance have been done. The analysis of isobaric peptides and avoiding charge competition effects in nano-ESI sources have been demonstrated.