Surface-Enhanced Raman Spectroscopic-Encoded Beads for Multiplex Immunoassay
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- 作者：Bong-Hyun Jun ; Jong-Ho Kim ; Hyunmi Park ; Jun-Sung Kim ; Kyeong-Nam Yu ; Sang-Myung Lee ; Heejeong Choi ; Seon-Yeong Kwak ; Yong-Kweon Kim ; Dae Hong Jeong ; Myung-Haing Cho ; Yoon-Sik Lee
- 刊名：Journal of Combinatorial Chemistry
- 出版年：2007
- 出版时间：March 2007
- 年：2007
- 卷：9
- 期：2
- 页码：237 - 244
- 全文大小：654K
- 年卷期：v.9,no.2(March 2007)
- ISSN：1520-4774
文摘
A new type of encoded bead, which uses surface-enhanced Raman scattering (SERS), is described formultiplex immunoassays. Silver nanoparticles were embedded in sulfonated polystyrene (PS) beads via apolyol method, and they were used as SERS-active substrates. Raman-label organic compounds such as4-methylbenzenethiol (4-MT), 2-naphthalenethiol (2-NT), and benzenethiol (BT) were then adsorbed ontothe silver nanoparticles in the sulfonated PS bead. Although only three kinds of encoding have beendemonstrated here, various combinations of these Raman-label organic compounds have the potential togive a large number of tags. The Raman-label-incorporated particles were then coated with a silica shellusing tetraethoxyorthosilicate (TEOS) for chemical stability and biocompatibility. The resulting beads showedunique and intense Raman signals for the labeled organic compounds. We demonstrated that SERS-encodedbeads could be used for multiplex detection with a model using streptavidin and p53. In our system, thebinding event of target molecules and the type of ligand can be simultaneously recognized by Ramanspectroscopy using a single laser-line excitation (514.5 nm).