Gene-Transferring Efficiencies of Novel Diamino Cationic Lipids with Varied Hydrocarbon Chains

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• 作者：Hong Sung Kim ; Jaeho Moon ; Keun Sik Kim ; Myung Min Choi ; Ji Eun Lee ; Yeon Heo ; Dae Hyan Cho ; Doo Ok Jang ; and Yong Serk Park
• 刊名：Bioconjugate Chemistry
• 出版年：2004
• 出版时间：September 2004
• 年：2004
• 卷：15
• 期：5
• 页码：1095 - 1101
• 全文大小：193K
• 年卷期：v.15,no.5(September 2004)
• ISSN：1520-4812

文摘

Utilizing three biocompatible components, a series of novel cationic lipids has been chemicallysynthesized and tested for their gene-transferring capabilities in 293 transformed kidney cells andB16BL6 mouse melanoma cells. The synthesized cationic lipids consisting of a core of lysine andaspartic acid with hydrocarbon chains of varied length were assigned the acronyms DLKD (O,O'-dilauryl N-lysylaspartate), DMKD (O,O'-dimyristyl N-lysylaspartate), DPKD (O,O'-dipalmityl N-lysylaspartate), and DSKD (O,O'-distearyl N-lysylaspartate). The gene-transferring capabilities ofthese cationic lipids were found to be dependent on the hydrocarbon chain length. Under similarexperimental conditions, the order of gene transfection efficiency was DMKD > DLKD > DPKD >DSKD. Addition of cholesterol or dioleoyl phosphatidylethanolamine (DOPE) as a colipid did not changethis order. Colipid addition affected the transfection efficiency positively or negatively depending onthe length of the cationic lipid acyl chain. On the whole, the length of the hydrophobic carbon chainwas a major factor governing the gene-transferring capabilities of this series of cationic lipids. Theobserved differences in transfection efficiency may be due to differing binding affinities to DNAmolecules as well as differences in the surface charge potential of the liposome-DNA complexes(lipoplexes) in the aqueous environment.