Hydrophilic Mesoporous Silica Materials for Highly Specific Enrichment of N-Linked Glycopeptide

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• 作者：Nianrong Sun ; Jiawen Wang ; Jizong Yao ; Chunhui Deng
• 刊名：Analytical Chemistry
• 出版年：2017
• 出版时间：February 7, 2017
• 年：2017
• 卷：89
• 期：3
• 页码：1764-1771
• 全文大小：550K
• ISSN：1520-6882

文摘

Hydrophilic interaction liquid chromatography (HILIC) is a significant enrichment strategy in glycoproteomics profiling. In this report, hydrophilic magnetic mesoporous silica materials (denoted as Fe₃O₄@mSiO₂-IDA) were designed and synthesized as an outstanding enrichment platform for glycopeptide analysis. By taking advantage of their merits, such as large surface area, excellent hydrophilicity and unbiased affinity toward all types of glycopeptides, the Fe₃O₄@mSiO₂-IDA nanomaterials were successfully applied to capture glycopeptides from complex samples. A total of 25 glycopeptides from horseradish peroxidase (HRP) digests and 33 glycopeptides from IgG were identified, respectively. Especially, as a result, 424 glycopeptides assigned to 140 glycoproteins were identified from only 2 μL human serum.