LC-MS/MS Analysis of Lysophospholipids Associated with Soy Protein Isolate

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• 作者：Nianbai Fang ; Shanggong Yu ; and Thomas M. Badger
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2003
• 出版时间：November 5, 2003
• 年：2003
• 卷：51
• 期：23
• 页码：6676 - 6682
• 全文大小：168K
• 年卷期：v.51,no.23(November 5, 2003)
• ISSN：1520-5118

文摘

Approximately 20-25% of babies consuming formula are fed formulas containing soy protein as thesole protein source. Isoflavone phytoestrogens are considered as major bioactive components presentin soy protein isolate (SPI) and the most widely studied phytochemicals in human diets and soyinfant formulas. In the present study, LC-MS/MS analysis of SPI phytochemical extract resulted inidentifications of fifty-six lysophospholipids (lyso-PL), including eighteen lysophosphatidylcholines (lyso-PC), twelve lysophosphatidylethanolamines (lyso-PE), eleven lysophosphatidylinositols (lyso-PI),eleven lysophosphatidic acids (lyso-PA) and four lysophosphatidylglycerols (lyso-PG). The LC-MS/MS conditions were first developed using commercially available standard mixtures. Under theseconditions, lyso-PL compounds could be separated by LC and yielded unique fragments and neutrallosses in the electrospray ionization mass spectrometry (ESI-MS) with both positive- and negative-ion modes, quite indicative of the polar headgroups for different subclasses. This is the firstcharacterization of lyso-PL in SPI. Lyso-PLs are important cell signaling and growth factor moleculesexperimentally linked to cardiovascular function and disease, cancer, and neurological disorders.These findings suggest that biological effects of PLs associated with SPI in infants clearly needs tobe considered as part of an overall evaluation of potential health benefits.Keywords: Soy protein isolate (SPI); SPI phytochemical; lysophospholipids; LC-MS/MS