Quantitative Raman Spectroscopy for the Analysis of Carrot Bioactives

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• 作者：Daniel P. Killeen ; Catherine E. Sansom ; Ross E. Lill ; Jocelyn R. Eason ; Keith C. Gordon ; Nigel B. Perry
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：March 20, 2013
• 年：2013
• 卷：61
• 期：11
• 页码：2701-2708
• 全文大小：390K
• 年卷期：v.61,no.11(March 20, 2013)
• ISSN：1520-5118

文摘

Rapid quantitative near-infrared Fourier transform Raman analyses of the key phytonutrients in carrots, polyacetylenes and carotenoids, are reported here for the first time. Solvent extracts of 31 carrot lines were analyzed for these phytonutrients by conventional methods, polyacetylenes by GC-FID and carotenoids by visible spectrophotometry. Carotenoid concentrations were 0鈥?586 渭g g^鈥? dry weight (DW). Polyacetylene concentrations were 74鈥?846 渭g g^鈥? DW, highest in wild carrots. The polyacetylenes were falcarinol, 6鈥?237 渭g g^鈥? DW; falcarindiol, 42鈥?475 渭g g^鈥? DW; and falcarindiol 3-acetate, 27鈥?49 渭g g^鈥? DW. Strong Raman bands for carotenoids gave good correlation to results by visible spectrophotometry. A chemometric model capable of quantitating carotenoids from Raman data was developed. A classification model for rapidly distinguishing carrots with high and low polyacetylene (limit of detection = 1400 渭g g^鈥?) concentrations based on Raman spectral intensity in the region of 2250 cm^鈥? was produced.<br>

Keywords:

Daucus; carrot; Raman; polyacetylenes; carotenoids; spectroscopy; chemometrics