文摘
We report the solvatochromic, viscosity-sensitive, and single-molecule photophysics of the fluorophoresDCDHF-N-6 and DCDHF-A-6. These molecules are members of the dicyanomethylenedihydrofuran (DCDHF)class of single-molecule emitters that contain an amine electron donor and a DCDHF acceptor linked by aconjugated unit; DCDHF-N-6 and DCDHF-A-6 have naphthalene- and anthracene-conjugated linkers,respectively. These molecules maintain the beneficial photophysics of the phenylene-linked DCDHF (i.e.,photostability, emission wavelength dependence on solvent polarity, and quantum yield sensitivity to solventviscosity), yet offer absorption and emission at longer wavelengths that are more appropriate for cellularimaging. We demonstrate that these new fluorophores are less photolabile in an aqueous environment thanseveral other commonly used dyes (rhodamine 6G, Texas Red, and fluorescein). Finally, we image singlecopies of the acene DCDHFs diffusing in the plasma membrane of living cells.