Stiffness Alterations of Single Cells Induced by UV in the Presence of NanoTiO2

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• 作者：Bertrand Vileno ; Ma<img src="http ; //pubs.acs.org/images/entities/lstrok.gif" border="0">gorzata Lekka ; Andrzej Sienkiewicz ; Sylvia Jeney ; Gabriela Stoessel ; Janusz Lekki ; Lá ; szl&oacute ; Forr&oac
• 刊名：Environmental Science & Technology
• 出版年：2007
• 出版时间：July 15, 2007
• 年：2007
• 卷：41
• 期：14
• 页码：5149 - 5153
• 全文大小：265K
• 年卷期：v.41,no.14(July 15, 2007)
• ISSN：1520-5851

文摘

Nanocrystalline titanium dioxide (nanoTiO₂) has beenreported to generate reactive oxygen species (ROS) underUV illumination. In our studies, changes in mechanicalproperties of human skin fibroblasts, exposed to the oxidativestress induced in the presence of nanoTiO₂ and UV light,were studied using atomic force microscopy (AFM).The exposure of cells to the action of ROS was performedat low TiO₂ concentration (4 images/entities/mgr.gif">g/mL) and under illuminationwith low-intensity UVA (8 and 20 mW/cm²) or UVC (0.1 mW/cm²). AFM measurements of the cell stiffness were carriedout immediately after exposure of cells to the oxidativestress. The data suggest that under illumination with low-intensity UVA nanoTiO₂ generates ROS, which, in turn,damage cellular and subcellular structures. This processwas detected by AFM as a marked drop in the cellular stiffnessof ca. 30-75%, which occurred rapidly, in the timeframe of 1 min. The photo-oxidative stress inducing thedecrease of cell stiffness was cancelled in the presenceof a well-established antioxidant, images/gifchars/beta2.gif" BORDER=0 ALIGN="middle">-carotene. The resultshighlight the sensitivity of AFM to detect early changes inmechanical properties of cells exposed to oxidativestress.