Dual Activity of Quinolinate Synthase: Triose Phosphate Isomerase and Dehydration Activities Play Together To Form Quinolinate
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- 作者：Debora Reichmann ; Yohann Cout茅 ; Sandrine Ollagnier de Choudens
- 刊名：Biochemistry
- 出版年：2015
- 出版时间：October 27, 2015
- 年：2015
- 卷：54
- 期：42
- 页码：6443-6446
- 全文大小：210K
- ISSN：1520-4995
文摘
Quinolinate synthase (NadA) is an Fe4S4 cluster-containing dehydrating enzyme involved in the synthesis of quinolinic acid (QA), the universal precursor of the essential coenzyme nicotinamide adenine dinucleotide. The reaction catalyzed by NadA is not well understood, and two mechanisms have been proposed in the literature that differ in the nature of the molecule (DHAP or G-3P) that condenses with iminoaspartate (IA) to form QA. In this article, using biochemical approaches, we demonstrate that DHAP is the triose that condenses with IA to form QA. The capacity of NadA to use G-3P is due to its previously unknown triose phosphate isomerase activity.