An IR-l
aser fluorin
ation technique is reported here for
an
alyzing the oxygen isotope composition (
ages/gifch
ars/delt
a.gif" BORDER=0 >
18O) of microscopic biogenic silic
a gr
ains (phytoliths
and di
atoms).Performed
after
a controlled isotopic exch
anged (CIE)procedure, the l
aser fluorin
ation technique th
at
allows
oneto visu
ally check the success of the fluorin
ation re
actionis f
aster th
an the convention
al fluorin
ation technique
and
allows
an
alyzing
ages/gifch
ars/delt
a.gif" BORDER=0 >
18O of sm
all to minute s
amples (1.6-0.3 mg)
as required for high-resolution p
aleoenvironment
al reconstructions. The long-term reproducibility
achievedwith the IR l
aser-he
ating fluorin
ation/O
2 ages/gifch
ars/delt
a.gif" BORDER=0 >
18O
an
alysis islower th
an or equ
al to ±0.26 (1 SD;
n = 99) forphytoliths
and ±0.17 (1 SD;
n = 47) for di
atoms. Whensever
al CIE
are t
aken into
account in the SD c
alcul
ation,the resulting reproducibility is lower th
an or equ
al to±0.51 for phytoliths (1 SD;
n = 99; CIE > 5)
and±0.54 (1 SD;
n = 47; CIE = 13) for di
atoms. Aminimum reproducibility of ±0.5 le
ads to
an estim
ateduncert
ainty on
ages/gifch
ars/delt
a.gif" BORDER=0 >
18O
silica close to ±0.5. Resulting uncert
ainties on reconstructed temper
ature
and
ages/gifch
ars/delt
a.gif" BORDER=0 >
18O
formingwater are, respectively, ±2
ages/entities/deg.gif">C
and ±0.5
and fit in theprecisions required for intertropic
al p
aleoenvironment
alreconstructions. Sever
al methodologic
al points such
asoptim
al extr
action protocols
and the necessity or not ofperforming two CIE prior to oxygen extr
action
are
assessed.