Glycated AAV Vectors: Chemical Redirection of Viral Tissue Tropism

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• 作者：Eric D. Horowitz ; Marc S. Weinberg ; Aravind Asokan
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：April 20, 2011
• 年：2011
• 卷：22
• 期：4
• 页码：529-532
• 全文大小：743K
• 年卷期：v.22,no.4(April 20, 2011)
• ISSN：1520-4812

文摘

A chemical approach for selective masking of arginine residues on viral capsids featuring an exogenous glycation reaction has been developed. Reaction of adeno-associated viral (AAV) capsids with the 伪-dicarbonyl compound, methylglyoxal, resulted in formation of arginine adducts. Specifically, surface-exposed guanidinium side chains were modified into charge neutral hydroimidazolones, thereby disrupting a continuous cluster of basic amino acid residues implicated in heparan sulfate binding. Consequent loss in heparin binding ability and decrease in infectivity were observed. Strikingly, glycated AAV retained the ability to infect neurons in the mouse brain and were redirected from liver to skeletal and cardiac muscle following systemic administration in mice. Further, glycated AAV displayed altered antigenicity demonstrating the potential for evading antibody neutralization. Generation of unnatural amino acid side chains through capsid glycation might serve as an orthogonal strategy to engineer AAV vectors displaying novel tissue tropisms for gene therapy applications.