Promotion of In Vitro Chondrogenesis of Mesenchymal Stem Cells Using In Situ Hyaluronic Hydrogel Functionalized with Rod-Like Viral Nanoparticles

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• 作者：Panita Maturavongsadit ; Jittima Amie Luckanagul ; Kamolrat Metavarayuth ; Xia Zhao ; Limin Chen ; Yuan Lin ; Qian Wang
• 刊名：Biomacromolecules
• 出版年：2016
• 出版时间：June 13, 2016
• 年：2016
• 卷：17
• 期：6
• 页码：1930-1938
• 全文大小：518K
• 年卷期：0
• ISSN：1526-4602

文摘

This study focuses on the development of injectable hydrogels to mimic the cartilage microenvironment using hyaluronic acid (HA) derivatives as starting materials. Cysteine-inserted Tobacco mosaic virus (TMV) mutants (TMV1cys) could be cross-linked to methacrylated hyaluronic acid (MeHA) polymers by thiol–ene “click” chemistry and form hydrogels under physiological condition. The resulting hydrogels could promote in vitro chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) significantly higher than that in the TMV-free HA hydrogels by upregulating bone morphogenetic protein-2 (BMP-2) expression and enhancing collagen accumulation.