On-Demand Self-Assembly of Supported Membranes Using Sacrificial, Anhydrobiotic Sugar Coats

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• 作者：Thomas E. Wilkop ; Jeremy Sanborn ; Ann E. Oliver ; Joshua M. Hanson ; Atul N. Parikh
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：January 8, 2014
• 年：2014
• 卷：136
• 期：1
• 页码：60-63
• 全文大小：255K
• ISSN：1520-5126

文摘

Borrowing principles of anhydrobiosis, we have developed a technique for self-assembling proteolipid-supported membranes on demand鈥攕imply by adding water. Intact lipid- and proteolipid vesicles dispersed in aqueous solutions of anhydrobiotic trehalose are vitrified on arbitrary substrates, producing glassy coats encapsulating biomolecules. Previous efforts establish that these carbohydrate coats arrest molecular mobilities and preserve native conformations and aggregative states of the embedded biomolecules, thereby enabling long-term storage. Subsequent rehydration, even after an extended period of time (e.g., weeks), devitrifies sugar鈥攔eleasing the cargo and unmasking the substrate surface鈥攖hus triggering substrate-mediated vesicle fusion in real time, producing supported membranes. Using this method, arrays of membranes, including those functionalized with membrane proteins, can be readily produced in situ by spatially addressing vitrification using common patterning tools鈥攗seful for multiplexed or stochastic sensing and assaying of target interactions with the fluid and functional membrane surface.