Production of L (+) Glutamic Acid in a Fully Membrane-Integrated Hybrid Reactor System: Direct and Continuous Production under Non-Neutralizing Conditions

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• 作者：D. Vikramachakravarthi ; Ramesh Kumar ; Parimal Pal
• 刊名：Industrial & Engineering Chemistry Research
• 出版年：2014
• 出版时间：December 10, 2014
• 年：2014
• 卷：53
• 期：49
• 页码：19019-19027
• 全文大小：401K
• ISSN：1520-5045

文摘

Experimental investigations were carried out on continuous and direct production of l-glutamic acid in a hybrid reactor system that integrated a conventional fermentative production step with downstream membrane-based separation and purification in flat sheet cross-flow membrane modules. To ensure constant fermentor capacity, the system was operated at steady state at different set flux values ranging from 76 L m^鈥? h^鈥? to 90 L m^鈥? h^鈥? at a dilution rate of 0.15 h^鈥?. Overcoming the substrate鈥損roduct inhibitions of traditional batch production system, this new, compact, flexible, and largely fouling-free design ensured steady and continuous production of l-glutamic acid directly from a renewable carbon source at the rate of about 8.4 g L^鈥? h^鈥?. Provisions of continuous product withdrawal, separation, and recycling of unconverted sugar and microbial cells ensured almost inhibition-free production under high cell concentration. Well-screened nanofiltration membranes with high selectivity helped achieve over 97% product purity while ensuring recovery and recycle of more than 95% unconverted carbon source resulting in a high yield of 0.95 g g^鈥?. The direct production scheme involves no phase change and use of harsh chemicals. The study presents development of a green process of glutamic acid production for sustainable business.