Oral Administration of Hyaluronan Reduces Bone Turnover in Ovariectomized Rats
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- 作者：Jenny Ma ; Patrick V. Granton ; David W. Holdsworth ; Eva A. Turley
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2013
- 出版时间：January 16, 2013
- 年：2013
- 卷：61
- 期：2
- 页码：339-345
- 全文大小：275K
- 年卷期：v.61,no.2(January 16, 2013)
- ISSN：1520-5118
文摘
The effect of oral hyaluronan (HA) on bone loss in ovariectomized (OVX) 3-month-old rats was measured using serum markers of bone turnover and bone mineral density. OVX rats were administered 1 mg/kg HA (OVX + HA) or phosphate-buffered saline (PBS) (OVX + PBS) by oral gavage (5 days/week for 54 days). Additional controls included sham ovariectomy with PBS gavage (Sham + PBS) and no treatment. Oral administration of HA resulted in approximately 50% (p < 0.05) increases in serum HA. Gel filtration analyses showed this was high molecular weight HA (300鈥?00 kDa). Osteopenia was mild due to the young age of the animals. Thus, ovariectomy resulted in a 30% increase in serum collagen N-terminal telopeptides (p < 0.001), a 20% increase in serum nitrate/nitrite levels (p = 0.05), and a 5鈥?% decrease in femur bone mineral density/content (p < 0.05). HA gavage blunted the development of osteopenia in this model as determined by preventing the 30% increase in serum collagen N-terminal telopeptide levels (p < 0.001) and by reducing bone mineral content loss from 6 to 4%. These results show that oral supplements of HA (gavage solution, 0.12% solution) significantly reduce bone turnover associated with mild osteopenia in rats.