A General Strategy for the Discovery of Metabolic Pathways: d-Threitol, l-Threitol, and Erythritol Utilization in Mycobacterium smegmatis

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• 作者：Hua Huang ; Michael S. Carter ; Matthew W. Vetting ; Nawar Al-Obaidi ; Yury Patskovsky ; Steven C. Almo ; John A. Gerlt
• 刊名：Journal of the American Chemical Society
• 出版年：2015
• 出版时间：November 25, 2015
• 年：2015
• 卷：137
• 期：46
• 页码：14570-14573
• 全文大小：276K
• ISSN：1520-5126

文摘

We describe a general integrated bioinformatic and experimental strategy to discover the in vitro enzymatic activities and in vivo functions (metabolic pathways) of uncharacterized enzymes discovered in microbial genome projects using the ligand specificities of the solute binding proteins (SBPs) for ABC transporters. Using differential scanning fluorimetry, we determined that the SBP for an ABC transporter encoded by the genome of Mycobacterium smegmatis is stabilized by d-threitol. Using sequence similarity networks and genome neighborhood networks to guide selection of target proteins for pathway enzymes, we applied both in vitro and in vivo experimental approaches to discover novel pathways for catabolism of d-threitol, l-threitol, and erythritol.