X-ray Photoelectron Spectroscopy- and Surface Plasmon Resonance-Detected Photo Release of Photolabile Protecting Groups from Nucleoside Self-Assembled Monolayers on Gold Surfaces

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• 作者：Katja Drexler ; Julia Smirnova ; Marina Galetskaya ; Snke Voss ; Mikhail Fonin ; Johannes Boneberg ; Ulrich Rdiger ; Paul Leiderer ; Ulrich E. Steiner
• 刊名：Langmuir
• 出版年：2009
• 出版时间：September 15, 2009
• 年：2009
• 卷：25
• 期：18
• 页码：10794-10801
• 全文大小：960K
• 年卷期：v.25,no.18(September 15, 2009)
• ISSN：1520-5827

文摘

The formation of self-assembled monolayers (SAMs) on gold by 2-(5-iodo-2-nitrophenyl) propoxycarbonyl (I-NPPOC)-protected thymidine with an attached mercaptohexyl succinate linker and the kinetics of photochemical release of the I-NPPOC group were monitored using X-ray photoelectron spectroscopy (XPS) and surface plasmon resonance (SPR) detection. In the XPS spectra, the iodine peaks allowed for specific and accurate monitoring of the presence and loss of I-NPPOC groups on the surface. In the SPR experiment, the overall signal change on photoillumination is in accord with a theoretical estimation of the density of I-NPPOC groups in a dense monolayer. The kinetics roughly follow a biexponential time dependence with two very different time constants, corresponding to photochemical quantum yields of 0.22 and 0.0032, respectively.