Synthesis and Immunogenicity of a Glycopolymer Conjugate

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• 作者：Tomasz Lipinski ; Pavel I. Kitov ; Adam Szpacenko ; Eugenia Paszkiewicz ; David R. Bundle
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：February 16, 2011
• 年：2011
• 卷：22
• 期：2
• 页码：274-281
• 全文大小：882K
• 年卷期：v.22,no.2(February 16, 2011)
• ISSN：1520-4812

文摘

A protective 尾-mannan trisaccharide epitope from the Candida albicans cell wall phosphomannan has been synthesized and activated for copolymerization with acrylamide. The resulting glycopolymer displayed 33 trisaccharide haptens and was derivatized for conjugation to the immunogenic carrier protein, chicken serum albumin. The resulting conjugate achieves a high degree of oligosaccharide substitution while limiting the sites of substitution on the protein. The murine immune response against this conjugate was compared with the response to a trisaccharide鈭抰etanus toxoid conjugate vaccine. The glycopolymer was shown to induce a more robust immune response with higher trisaccharide-specific antibody titers and with a significantly larger proportion of responding mice developing antibodies that bound the target, native cell wall antigen of C. albicans.