Identification of Echinacoside Metabolites Produced by Human Intestinal Bacteria Using Ultraperformance Liquid Chromatography鈥換uadrupole Time-of-Flight Mass Spectrometry

详细信息    查看全文

• 作者：Yang Li ; Guisheng Zhou ; Shihua Xing ; Pengfei Tu ; Xiaobo Li
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：August 5, 2015
• 年：2015
• 卷：63
• 期：30
• 页码：6764-6771
• 全文大小：395K
• ISSN：1520-5118

文摘

Echinacoside (ECH) is one of the representative phenylethanoid glycosides. It is widely present in plants and exhibits various bioactivities. However, the extremely low oral bioavailability of ECH in rats implies that ECH may go through multiple hydrolysis steps in the gastrointestinal tract prior to its absorption into the blood. Therefore, the gastrointestinal metabolites of ECH are more likely to be the bioactive components. This study established an approach combining ultraperformance liquid chromatography鈥搎uadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) with MS^E technology and MetaboLynx software for rapid analysis of the ECH metabolic profile produced by human intestinal bacteria. As a result, 13 ECH metabolites and 5 possible metabolic pathways (including deglycosylation, dehydroxylation, reduction, hydroxylation, and acetylation) were identified. Furthermore, hydroxytyrosol (HT) and 3-hydroxyphenylpropionic acid (3-HPP) were found to be the two bioactive metabolites of ECH produced by human intestinal bacteria.