Off-Line Coupling of Capillary Electrophoresis to Substrate-Assisted Laser Desorption Inductively Coupled Plasma Mass Spectrometry

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• 作者：Ondr&#780 ; ej Pes&#780 ; Pavla Jungová ; Radek Vyhná ; nek ; Tomá ; s&#780 ; Vaculovic&#780 ; Viktor Kanický ; Jan Preisler
• 刊名：Analytical Chemistry
• 出版年：2008
• 出版时间：November 15, 2008
• 年：2008
• 卷：80
• 期：22
• 页码：8725-8732
• 全文大小：162K
• 年卷期：v.80,no.22(November 15, 2008)
• ISSN：1520-6882

文摘

A novel off-line coupling of capillary electrophoresis (CE) and inductively coupled plasma mass spectrometry (ICPMS) is reported here. The coupling interface is based on the connection of a separation capillary to a deposition capillary via a liquid junction maintaining high separation efficiency and sample utilization due to the self-focusing effect and lack of pressure-induced flow in comparison with nebulizer-like interfaces. The separation is recorded in the form of droplets of CE effluent on a suitable substrate  a poly(ethylene terephthalate) glycol (PETG) sample plate placed inside a partially evacuated chamber. Substrate-assisted laser desorption (SALD) is used to vaporize the sample fractions and to enable further transfer to the ICPMS. The mechanism of SALD is examined using model samples deposited on a variety of substrates. The highest response is obtained for a PETG substrate; sample desorption due to ablation of PETG is found to outweigh direct ablation of sample. Detection limits are given for several metal elements. Finally, a rapid (2.5-min), high-resolution separation of Cr^III/Cr^VI species injected in subpicomolar quantity is shown.