Proteomic Discovery and Development of a Multiplexed Targeted MRM-LC-MS/MS Assay for Urine Biomarkers of Extracellular Matrix Disruption in Mucopolysaccharidoses I, II, and VI

详细信息    查看全文

• 作者：Wendy E. Heywood ; Stephane Camuzeaux ; Ivan Doykov ; Nina Patel ; Rhian-Lauren Preece ; Emma Footitt ; Maureen Cleary ; Peter Clayton ; Stephanie Grunewald ; Lara Abulhoul ; Anupam Chakrapani ; Neil J. Sebire ; Peter Hindmarsh ; Tom J. de Koning ; Simon Heales ; Derek Burke ; Paul Gissen ; Kevin Mills
• 刊名：Analytical Chemistry
• 出版年：2015
• 出版时间：December 15, 2015
• 年：2015
• 卷：87
• 期：24
• 页码：12238-12244
• 全文大小：368K
• ISSN：1520-6882

文摘

The mucopolysaccharidoses (MPS) are lysosomal storage disorders that result from defects in the catabolism of glycosaminoglycans. Impaired muscle, bone, and connective tissue are typical clinical features of MPS due to disruption of the extracellular matrix. Markers of MPS disease pathology are needed to determine disease severity and monitor effects of existing and emerging new treatments on disease mechanisms. Urine samples from a small cohort of MPS-I, -II, and -VI patients (n = 12) were analyzed using label-free quantative proteomics. Fifty-three proteins including many associated with extracellular matrix organization were differently expressed. A targeted multiplexed peptide MRM LC-MS/MS assay was used on a larger validation cohort of patient samples (MPS-I n = 18, MPS-II n = 12, MPS-VI n = 6, control n = 20). MPS-I and -II groups were further subdivided according to disease severity. None of the markers assessed were altered significantly in the mild disease groups compared to controls. 尾-galactosidase, a lysosomal protein, was elevated 3.6鈥?.7-fold significantly (p < 0.05) in all disease groups apart from mild MPS-I and -II. Collagen type I伪, fatty-acid-binding-protein 5, nidogen-1, cartilage oligomeric matrix protein, and insulin-like growth factor binding protein 7 concentrations were elevated in severe MPS I and II groups. Cartilage oligomeric matrix protein, insulin-like growth factor binding protein 7, and 尾-galactosidase were able to distinguish the severe neurological form of MPS-II from the milder non-neurological form. Protein Heg1 was significantly raised only in MPS-VI. This work describes the discovery of new biomarkers of MPS that represent disease pathology and allows the stratification of MPS-II patients according to disease severity.