Induction of Apoptosis by 3-Amino-6-(3-aminopropyl)-5,6-dihydro-5,11-dioxo-11H-indeno[1,2-c]isoquinoline via Modulation of MAPKs (p38 and c-Jun N-terminal Kinase) and c-Myc in HL-

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• 作者：Eun-Jung Park ; Evgeny Kiselev ; Martin Conda-Sheridan ; Mark Cushman ; John M. Pezzuto
• 刊名：Journal of Natural Products
• 出版年：2012
• 出版时间：March 23, 2012
• 年：2012
• 卷：75
• 期：3
• 页码：378-384
• 全文大小：360K
• 年卷期：v.75,no.3(March 23, 2012)
• ISSN：1520-6025

文摘

Recently, we reported that 3-amino-6-(3-aminopropyl)-5,6-dihydro-5,11-dioxo-11H-indeno[1,2-c]isoquinoline (AM6-36), sharing structural similarity with naturally occurring isoquinolines, induced activities mediated by retinoid X receptor (RXR) response element accompanied by antiproliferative effects on breast cancer cells. To further characterize the biologic potential of AM6-36, we currently report studies conducted with HL-60 human leukemia cells. AM6-36 significantly inhibited cellular proliferation in a dose- and time-dependent manner with an IC₅₀ value of 86 nM. When evaluated at low test concentrations (鈮?.25 渭M), AM6-36 induced arrest in the G2/M phase of the cell cycle. At higher concentrations (1 and 2 渭M), the response shifted to apoptosis, which was consistent with the effect of AM6-36 on other apoptotic signatures including an increase of apoptotic annexin V⁺ 7-AAD^{鈥?/sup> cells, loss of mitochondrial membrane potential, induction of poly(ADP-ribose) polymerase cleavage, and activation of several caspases. These apoptotic effects are potentially due to up-regulation of p38 MAPK and JNK phosphorylation and down-regulation of c-Myc oncogene expression. Taken together, AM6-36 might serve as an effective anticancer agent by inducing G2/M cell cycle arrest and apoptosis through the activation of MAPKs and inhibition of c-Myc.}