Nickel(II)-Induced Excimer Formation of a Naphthalene-Based Fluorescent Probe for Living Cell Imaging

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• 作者：Arnab Banerjee ; Animesh Sahana ; Subarna Guha ; Sisir Lohar ; Ipsit Hauli ; Subhra Kanti Mukhopadhyay ; Jes煤s Sanmart铆n Matalobos ; Debasis Das
• 刊名：Inorganic Chemistry
• 出版年：2012
• 出版时间：May 21, 2012
• 年：2012
• 卷：51
• 期：10
• 页码：5699-5704
• 全文大小：416K
• 年卷期：v.51,no.10(May 21, 2012)
• ISSN：1520-510X

文摘

Ni²⁺-induced intramolecular excimer formation of a naphthalene-based novel fluorescent probe, 1-[(naphthalen-3-yl)methylthio]-2-[(naphthalen-6-yl)methylthio]ethane (L), has been investigated for the first time and nicely demonstrated by excitation spectra, a fluorescence lifetime experiment, and ¹H NMR titration. The addition of Ni²⁺ to a solution of L (DMSO:water = 1:1, v/v; 位_em = 345 nm, 位_ex = 280 nm) quenched its monomer emission, with subsequent enhancement of the excimer intensity (at 430 nm) with an isoemissive point at 381 nm. The fluorescence lifetime of free L (0.3912 ns) is much lower than that of the nickel(2+) complex (1.1329 ns). L could detect Ni²⁺ as low as 1 脳 10^鈥? M with a fairly strong binding constant, 2.0 脳 10⁴ M^鈥?. Ni²⁺-contaminated living cells of plant origin could be imaged using a fluorescence microscope.