Novel Biodegradable Aliphatic Poly(butylene Succinate-co-cyclic carbonate)s Bearing Functionalizable Carbonate Building Blocks: II. Enzymatic Biodegradation and in Vitro Biocompatibility Assay

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• 作者：Jing Yang ; Weisheng Tian ; Qiaobo Li ; Yang Li ; and Amin Cao
• 刊名：Biomacromolecules
• 出版年：2004
• 出版时间：November 2004
• 年：2004
• 卷：5
• 期：6
• 页码：2258 - 2268
• 全文大小：462K
• 年卷期：v.5,no.6(November 2004)
• ISSN：1526-4602

文摘

In a previous study, we have reported chemical synthesis of novel aliphatic poly(butylene succinate-co-cyclic carbonate) P(BS-co-CC)s bearing various functionalizable carbonate building blocks, and this workwill continue to present our new studies on their enzymatic degradation and in vitro cell biocompatibilityassay. First, enzymatic degradation of the novel P(BS-co-CC) film samples was investigated with two enzymesof lipase B Candida Antartic (Novozyme 435) and lipase Porcine Pancreas PPL, and it was revealed thatcopolymerizing linear poly(butylene succinate) PBS with a functionalizable carbonate building block couldremarkably accelerate the enzymatic degradation of a synthesized product P(BS-co-CC), and its biodegradationbehavior was found to strongly depend on the overall impacts of several important factors as the cycliccarbonate (CC) comonomer structure and molar content, molar mass, thermal characteristics, morphology,the enzyme-substrate specificity, and so forth. Further, the biodegraded residual film samples and water-soluble enzymatic degradation products were allowed to be analyzed by means of proton nuclear magneticresonance (¹H NMR), gel permeation chromatograph (GPC), differential scanning calorimeter (DSC),attenuated total reflection FTIR (ATR-FTIR), scanning electron microscope (SEM), and liquid chromatograph-mass spectrometry (LC-MS). On the experimental evidences, an exo-type mechanism of enzymatic chainhydrolysis preferentially occurring in the noncrystalline domains was suggested for the synthesized newP(BS-co-CC) film samples. With regard to their cell biocompatibilities, an assay with NIH 3T3 mousefibroblast cell was conducted using the novel synthesized P(BS-co-CC) films as substrates with respect tothe cell adhesion and proliferation, and these new biodegradable P(BS-co-CC) samples were found to exhibitas low cell toxicity as the PLLA control, particularly the two samples of poly(butylene succinate-co-18.7mol % dimethyl trimethylene carbonate) P(BS-co-18.7 mol % DMTMC) and poly(butylene succinate-co-21.9 mol % 5-benzyloxy trimethylene carbonate) P(BS-co-21.9 mol % BTMC) were interestingly found toshow much better cell biocompatibilities than the PLLA reference.