Unnatural Amino Acid Incorporation into Virus-Like Particles

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• 作者：Erica Strable ; Duane E. Prasuhn Jr. ; Andrew K. Udit ; Steven Brown ; A. James Link ; John T. Ngo ; Gabriel Lander ; Joel Quispe ; Clinton S. Potter ; Bridget Carragher ; David A. Tirrell ; M. G. Finn
• 刊名：Bioconjugate Chemistry
• 出版年：2008
• 出版时间：April 2008
• 年：2008
• 卷：19
• 期：4
• 页码：866 - 875
• 全文大小：1618K
• 年卷期：v.19,no.4(April 2008)
• ISSN：1520-4812

文摘

Virus-like particles composed of hepatitis B virus (HBV) or bacteriophage Qb2; capsid proteins have been labeled with azide- or alkyne-containing unnatural amino acids by expression in a methionine auxotrophic strain of E. coli. The substitution does not affect the ability of the particles to self-assemble into icosahedral structures indistinguishable from native forms. The azide and alkyne groups were addressed by Cu(I)-catalyzed [3 + 2] cycloaddition: HBV particles were decomposed by the formation of more than 120 triazole linkages per capsid in a location-dependent manner, whereas Qb2; suffered no such instability. The marriage of these well-known techniques of sense-codon reassignment and bioorthogonal chemical coupling provides the capability to construct polyvalent particles displaying a wide variety of functional groups with near-perfect control of spacing.