Mulberry 1-Deoxynojirimycin Inhibits Adipogenesis by Repression of the ERK/PPAR纬 Signaling Pathway in Porcine Intramuscular Adipocytes

详细信息    查看全文

• 作者：Guo-qiang Wang ; Li Zhu ; Mei-lin Ma ; Xiao-chang Chen ; Yun Gao ; Tai-yong Yu ; Gong-she Yang ; Wei-jun Pang
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2015
• 出版时间：July 15, 2015
• 年：2015
• 卷：63
• 期：27
• 页码：6212-6220
• 全文大小：500K
• ISSN：1520-5118

文摘

Intramuscular fat (IMF), which is modulated by adipogenensis of intramuscular adipocytes, plays a key role in pork quality associated with marbling, juiceness, and flavor. However, the regulatory mechanism of 1-deoxynojirimycin (DNJ) on adipogenesis is still unknown. Here, we found that both DNJ (2.0, 3.0, 4.0, 5.0, and 6.0 渭M) and rosiglitazone (RSG; 0.1, 0.2, 0.3, 0.4, and 0.5 mM) had no effect on cell viability. Moreover, 4 渭M DNJ significantly inhibited adipogenesis, whereas 0.4 mM RSG increased lipogenesis of porcine intramuscular adipocytes. Interestingly, DNJ sharply inhibited phosphorylation of extracellular regulated protein kinases 1/2 (ERK1/2), but did not change phosphorylation of AKT (protein kinase B) in intramuscular adipocytes. We further found that the inhibitory adipogenesis of DNJ was attenuated by RSG via up-regulation of PPAR纬. On the basis of the above findings, we suggest that DNJ inhibited adipogenesis through the ERK/PPAR纬 signaling pathway in porcine intramuscular adipocytes.