Fluorescence Lifetime Imaging of Nanoflares for mRNA Detection in Living Cells

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• 作者：Jing Shi ; Ming Zhou ; Aihua Gong ; Qijun Li ; Qian Wu ; Gary J. Cheng ; Mingyang Yang ; Yaocheng Sun
• 刊名：Analytical Chemistry
• 出版年：2016
• 出版时间：February 16, 2016
• 年：2016
• 卷：88
• 期：4
• 页码：1979-1983
• 全文大小：304K
• ISSN：1520-6882

文摘

The expression level of tumor-related mRNA can reveal significant information about tumor progression and prognosis, so specific mRNA in cells provides an important approach for biological and disease studies. Here, fluorescence lifetime imaging of nanoflares in living cells was first employed to detect specific intracellular mRNA. We characterized the lifetime changes of the prepared nanoflares before and after the treatment of target mRNA and also compared the results with those of fluorescence intensity-based measurements both intracellularly and extracellularly. The nanoflares released the cy5-modified oligonucleotides and bound to the targets, resulting in a fluorescence lifetime lengthening. This work puts forward another dimension of detecting specific mRNA in cells and can also open new ways for detection of many other biomolecules.