Tunneling and Coupled Motion in the Escherichia coli Dihydrofolate Reductase Catalysis

详细信息    查看全文

• 作者：R. Steven Sikorski ; Lin Wang ; Kelli A. Markham ; P. T. Ravi Rajagopalan ; Stephen J. Benkovic ; and Amnon Kohen
• 刊名：Journal of the American Chemical Society
• 出版年：2004
• 出版时间：April 21, 2004
• 年：2004
• 卷：126
• 期：15
• 页码：4778 - 4779
• 全文大小：33K
• 年卷期：v.126,no.15(April 21, 2004)
• ISSN：1520-5126

文摘

H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1tities/deg.gif"> to 2tities/deg.gif"> coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.