Fast Quantitative Method for the Analysis of Carotenoids in Transgenic Maize

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• 作者：Sol Maiam Rivera ; Francisca Vilar贸 ; Changfu Zhu ; Chao Bai ; Gemma Farr茅 ; Paul Christou ; Ramon Canela-Garayoa
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2013
• 出版时间：June 5, 2013
• 年：2013
• 卷：61
• 期：22
• 页码：5279-5285
• 全文大小：355K
• 年卷期：v.61,no.22(June 5, 2013)
• ISSN：1520-5118

文摘

A fast method was developed to determine carotenoid content in transgenic maize seeds. The analysis was carried out using an ultrahigh-pressure liquid chromatograph coupled to a photodiode array detector and a mass spectrometer (UHPLC-PDA-MS/MS). Sixteen carotenoid pigments were detected and quantified in <13 min. In addition, it was possible to obtain good resolution of both polar xanthophylls and nonpolar carotenes. The method exhibited (a) a high degree of repeatability (%RSD < 13%), (b) linear calibration curves (R² > 0.9952), (c) satisfactory recoveries for most of the pigments (between 82 and 108%), and (d) low detection (from 0.02 to 0.07 渭g/mL) and quantification limits (from 0.05 to 0.20 渭g/mL) (LOD and LOQ, respectively). The methodology was applied to the analysis of transgenic maize lines TM1, TM2, and TM3, expressing several carotenogenic genes.

Keywords:

maize; carotenoids; ultrahigh-pressure liquid chromatography (UHPLC)