Chemical Mechanism of the Phosphotriesterase from Sphingobium sp. Strain TCM1, an Enzyme Capable of Hydrolyzing Organophosphate Flame Retardants

详细信息    查看全文

• 作者：Andrew N. Bigley ; Dao Feng Xiang ; Zhongjie Ren ; Haoran Xue ; Kenneth G. Hull ; Daniel Romo ; Frank M. Raushel
• 刊名：Journal of the American Chemical Society
• 出版年：2016
• 出版时间：March 9, 2016
• 年：2016
• 卷：138
• 期：9
• 页码：2921-2924
• 全文大小：281K
• ISSN：1520-5126

文摘

The mechanism of action of the manganese-dependent phosphotriesterase from Sphingobium sp. strain TCM1 that is capable of hydrolyzing organophosphate flame retardants was determined. The enzyme was shown to hydrolyze the R_P-enantiomer of O-methyl O-cyclohexyl p-nitrophenyl thiophosphate with net inversion of configuration and without the formation of a covalent reaction intermediate. These results demonstrate that the enzyme catalyzes the hydrolysis of substrates by activation of a nucleophilic water molecule for direct attack at the phosphorus center.