Two-Dimensional Liquid Chromatography鈥揟andem Mass Spectrometry Coupled with Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Labeling Approach Revealed First Proteome Profiles of Pulmona
详细信息 查看全文
- 作者：Qi Lu ; Juan Bai ; Lili Zhang ; Jie Liu ; Zhihua Jiang ; Jennifer J. Michal ; Qindong He ; Ping Jiang
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：May 4, 2012
- 年：2012
- 卷：11
- 期：5
- 页码：2890-2903
- 全文大小：593K
- 年卷期：v.11,no.5(May 4, 2012)
- ISSN：1535-3907
文摘
Porcine reproductive and respiratory syndrome (PRRS) has devastated the pig industry worldwide for almost 25 years, and its virus (PRRSV) preferentially infects and replicates in pulmonary alveolar macrophages (PAMs). To discover cellular protein responses in PRRSV-infected PAMs, two-dimensional liquid chromatography鈥搕andem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling was employed to quantitatively identify the differentially expressed proteins between the PRRSV-infected groups and the controls. A total of 160 cellular proteins in PAMs that were significantly altered post-infection were identified. These differentially expressed proteins are related to the biological processes of virus binding, cell structure, signal transduction, cell adhesion, etc., and their interactions. This is the first report that analyzed the cellular protein profile of PRRSV-infected PAMs using iTRAQ technology, and this data provides important information to help understand the host response to PRRSV and to define the cellular requirements for the underlying mechanism of PRRSV replication and pathogenesis.