Isolation and Identification of Phlorotannins from Ecklonia stolonifera with Antioxidant and Anti-inflammatory Properties

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• 作者：A-Reum Kim ; Tai-Sun Shin ; Min-Sup Lee ; Ji-Young Park ; Kyoung-Eun Park ; Na-Young Yoon ; Jong-Soon Kim ; Jae-Sue Choi ; Byeong-Churl Jang ; Dae-Seok Byun ; Nam-Kyu Park ; Hyeung-Rak Kim
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2009
• 出版时间：May 13, 2009
• 年：2009
• 卷：57
• 期：9
• 页码：3483-3489
• 全文大小：854K
• 年卷期：v.57,no.9(May 13, 2009)
• ISSN：1520-5118

文摘

Bioactivity-guided fractionation of Ecklonia stolonifera was used to determine the chemical identity of bioactive constituents, with potent antioxidant activities. The structures of the phlorotannins were determined on the basis of spectroscopic analysis, including NMR and mass spectrometry analysis. The antioxidant activities of the isolated compounds were evaluated by free radical scavenging activities in both in vitro and cellular systems. The anti-inflammatory effects of the isolated compounds were evaluated by determining their inhibitory effects on the production of nitric oxide (NO) and prostaglandin E₂ (PGE₂) in lipopolysaccharide (LPS)-induced RAW 264.7 murine macrophage cells. The results indicated that phlorofucofuroeckol A, dieckol, and dioxinodehydroeckol showed potential radical scavenging activities against 2,2-diphenyl-1-picrylhydrazyl. Among them, phlorofucofuroeckol A and dieckol significantly suppressed the intracellular reactive oxygen species level assayed by 2′,7′-dichlorofluorescein diacetate assay in LPS-induced RAW 264.7 cells. Phlorofucofuroeckol A significantly inhibited the LPS-induced production of NO and PGE₂ through the down-regulation of inducible nitric oxide synthase and cyclooxygenase 2 protein expressions. In conclusion, these results suggest that phlorofucofuroeckol A has a potential for functional foods with antioxidant and anti-inflammatory activities.