Characterization of the Terminal Activation Step Catalyzed by Oxygenase CmmOIV of the Chromomycin Biosynthetic Pathway from Streptomyces griseus

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• 作者：Mary A. Bosserman ; Ana B. Flo虂rez ; Khaled A. Shaaban ; Alfredo F. Bran虄a ; Jose A. Salas ; Carmen Me虂ndez ; Ju虉rgen Rohr
• 刊名：Biochemistry
• 出版年：2011
• 出版时间：March 1, 2011
• 年：2011
• 卷：50
• 期：8
• 页码：1421-1428
• 全文大小：926K
• 年卷期：v.50,no.8(March 1, 2011)
• ISSN：1520-4995

文摘

Inactivation and initial interrogation of key oxygenase CmmOIV of the biosynthetic pathway of chromomycin A₃ in Streptomyces griseus ssp. griseus revealed that a completely methylated and acetylated prechromomycin is the preferred substrate of this enzyme. This suggests that the three sugar decoration reactions, two O-acetylations and an O-methylation, which were previously believed to occur as the final steps of chromomycin A₃ biosynthesis, indeed take place prior to the CmmOIV reaction. Upon inactivation of CmmOIV, four new compounds accumulated; the fully decorated prechromomycin and its incompletely acetylated precursor along with a diketoprechromomycin-type compound were fully characterized and assayed with CmmOIV.