Characterization of a Radiation-Induced Stress Response Communicated in Vivo between Zebrafish

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• 作者：Carmel Mothersill ; Richard W. Smith ; Nalini Agnihotri ; Colin B. Seymour
• 刊名：Environmental Science & Technology
• 出版年：2007
• 出版时间：May 1, 2007
• 年：2007
• 卷：41
• 期：9
• 页码：3382 - 3387
• 全文大小：306K
• 年卷期：v.41,no.9(May 1, 2007)
• ISSN：1520-5851

文摘

Radiation-induced communication of stress signalsbetween rainbow trout (Oncorhynchus mykiss W) haverecently been described by this group and linked to thebystander effect. This paper addresses the question of whetheranother totally unrelated fish species (Danio rerio L) candemonstrate the effect and also looks at attenuation of boththe bystander signal, from irradiated fish, and the bystandereffect, in naive fish. The data show that zebrafish producebystander signals, and that, as with rainbow trout these canaffect naïve (i.e., non-irradiated) fish placed in waterwith X-rayed fish or in water previously occupied by X-rayedfish. Skin explants from directly X-rayed fish still reduceHPV-G reporter cell growth 6 h after X-ray, but the bystandersignal to naïve fish is lost. Twelve h after X-ray thesignal is lost in X-rayed fish. The bystander effect is alsoattenuated if induction was by placing naïve fish inwater which previously held the X-rayed fish. However,the effect is retained if induction was by placing X-rayedand naïve fish together. This suggests the signal is notretained by water for long periods of time. Individual fishdata reveal unique responses by bystander fish which couldindicate varying levels of sensitivity to signal strengthamong individuals.