Development of PLGA-Mannosamine Nanoparticles as Oral Protein Carriers

详细信息    查看全文

• 作者：Maria Alonso-Sande ; Anne des Rieux ; Virginie Fievez ; Bruno Sarmento ; Araceli Delgado ; Carmen Evora ; Carmen Remu帽谩n-L贸pez ; V茅ronique Pr茅at ; Maria J. Alonso
• 刊名：Biomacromolecules
• 出版年：2013
• 出版时间：November 11, 2013
• 年：2013
• 卷：14
• 期：11
• 页码：4046-4052
• 全文大小：334K
• 年卷期：v.14,no.11(November 11, 2013)
• ISSN：1526-4602

文摘

Here we report the development of polymeric nanoparticles, made of poly(lactide-co-glycolide) (PLGA) chemically modified with mannosamine (MN), intended to specifically interact with the intestinal mucosa and facilitate the intestinal transport of proteins. PLGA-MN nanoparticles displayed nanometric size and a negative zeta potential, which was lower than that of the PLGA nanoparticles. This correlate well with the preferential location of the MN group on the nanoparticles surface obtained by X-ray photoelectron spectroscope (XPS). The presence of MN groups in the polymer chain led to a different surface morphology noted by SEM, an increase of the encapsulation of model proteins, and to help stabilizing the nanoparticles in simulated intestinal fluids. Furthermore, the MN modification significantly enhanced the nanoparticle鈥檚 interaction with the epithelial cells in human intestinal follicle-associated epithelium cell culture model. Overall, the MN modification significantly modifies the properties of PLGA nanoparticles making them more suitable as nanocarriers for oral protein delivery.