Cytotoxicity of Carbon Nanomaterials: Single-Wall Nanotube, Multi-Wall Nanotube, and Fullerene
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- 作者：Guang Jia ; Haifang Wang ; Lei Yan ; Xiang Wang ; Rongjuan Pei ; Tao Yan ; Yuliang Zhao ; and Xinbiao Guo
- 刊名：Environmental Science & Technology
- 出版年：2005
- 出版时间：March 1, 2005
- 年：2005
- 卷：39
- 期：5
- 页码：1378 - 1383
- 全文大小：733K
- 年卷期：v.39,no.5(March 1, 2005)
- ISSN：1520-5851
文摘
A cytotoxicity test protocol for single-wall nanotubes(SWNTs), multi-wall nanotubes (with diameters rangingfrom 10 to 20 nm, MWNT10), and fullerene (C60) was tested.Profound cytotoxicity of SWNTs was observed in alveolarmacrophage (AM) after a 6-h exposure in vitro. Thecytotoxicity increases by as high as ~35% when the dosageof SWNTs was increased by 11.30 
g/cm2. No significanttoxicity was observed for C60 up to a dose of 226.00g/cm2. The cytotoxicity apparently follows a sequenceorder on a mass basis: SWNTs > MWNT10 > quartz >C60. SWNTs significantly impaired phagocytosis of AM at thelow dose of 0.38 g/cm2, whereas MWNT10 and C60induced injury only at the high dose of 3.06 g/cm2. Themacrophages exposed to SWNTs or MWNT10 of 3.06g/cm2 showed characteristic features of necrosis anddegeneration. A sign of apoptotic cell death likely existed.Carbon nanomaterials with different geometric structuresexhibit quite different cytotoxicity and bioactivity in vitro,although they may not be accurately reflected in thecomparative toxicity in vivo.
g/cm2. No significanttoxicity was observed for C60 up to a dose of 226.00g/cm2. The cytotoxicity apparently follows a sequenceorder on a mass basis: SWNTs > MWNT10 > quartz >C60. SWNTs significantly impaired phagocytosis of AM at thelow dose of 0.38 g/cm2, whereas MWNT10 and C60induced injury only at the high dose of 3.06 g/cm2. Themacrophages exposed to SWNTs or MWNT10 of 3.06g/cm2 showed characteristic features of necrosis anddegeneration. A sign of apoptotic cell death likely existed.Carbon nanomaterials with different geometric structuresexhibit quite different cytotoxicity and bioactivity in vitro,although they may not be accurately reflected in thecomparative toxicity in vivo.