A Comprehensive Proteome of Mycoplasma genitalium
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- 作者：Noem铆 P谩rraga-Ni帽o ; Nuria Colom茅-Calls ; Francesc Canals ; Enrique Querol ; Mario Ferrer-Navarro
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：June 1, 2012
- 年：2012
- 卷：11
- 期：6
- 页码：3305-3316
- 全文大小：440K
- 年卷期：v.11,no.6(June 1, 2012)
- ISSN：1535-3907
文摘
Mycoplasma genitalium is a human pathogen associated with several sexually transmitted diseases. Proteomic technologies, along with other methods for global gene expression analysis, play a key role in understanding the mechanisms of bacterial pathogenesis and physiology. The proteome of M. genitalium, model of a minimal cell, has been extended using a combination of different proteomic approaches and technologies. The total proteome of this microorganism has been analyzed using gel-based and gel-free approaches, achieving the identification of 85.3% of the predicted ORFs. In addition, a comprehensive analysis of membrane subproteome has been performed. For this purpose, the TX-114 soluble fraction has been analyzed as well as the surface proteins, using cell-surface protein labeling with CyDye. Finally, the serological response of M. genitalium-infected patients and healthy donors has been analyzed to identify proteins that trigger immunological response. Here, we present the most extensive M. genitalium proteome analysis (85.3% of predicted ORFs), a comprehensive M. genitalium membrane analysis, and a study of the human serological response to M. genitalium.