Phylogenetic Analysis of TCE-Dechlorinating Consortia Enriched on a Variety of Electron Donors
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- 作者：Ryan A. Freeborn ; Kimberlee A. West ; Vishvesh K. Bhupathiraju ; Sadhana Chauhan ; Brian G. Rahm ; Ruth E. Richardson ; and Lisa Alvarez-Cohen
- 刊名：Environmental Science & Technology
- 出版年：2005
- 出版时间：November 1, 2005
- 年：2005
- 卷：39
- 期：21
- 页码：8358 - 8368
- 全文大小：262K
- 年卷期：v.39,no.21(November 1, 2005)
- ISSN：1520-5851
文摘
Two rapidly fermented electron donors, lactate andmethanol, and two slowly fermented electron donors,propionate and butyrate, were selected for enrichmentstudies to evaluate the characteristics of anaerobic microbialconsortia that reductively dechlorinate TCE to ethene.Each electron donor enrichment subculture demonstratedthe ability to dechlorinate TCE to ethene through severalserial transfers. Microbial community analyses based upon16S rDNA, including terminal restriction fragment lengthpolymorphism (T-RFLP) and clone library/sequencing, wereperformed to assess major changes in microbial communitystructure associated with electron donors capable ofstimulating reductive dechlorination. Results demonstratedthat five phylogenic subgroups or genera of bacteriawere present in all consortia, including Dehalococcoidessp., low G+C Gram-positives (mostly Clostridium andEubacterium sp.), Bacteroides sp., Citrobacter sp., and 
Proteobacteria (mostly Desulfovibrio sp.). Phylogeneticassociation indicates that only minor shifts in the microbialcommunity structure occurred between the four alternateelectron donor enrichments and the parent consortium.Inconsistent detection of Dehalococcoides spp. in clonelibraries and T-RFLP of enrichment subcultures wasresolved using quantitative polymerase chain reaction (Q-PCR). Q-PCR with primers specific to Dehalococcoides16S rDNA resulted in positive detection of this species inall enrichments. Our results suggest that TCE-dechlorinatingconsortia can be stably maintained on a variety of electrondonors and that quantities of Dehalococcoides cellsdetected with Dehalococcoides specific 16S rDNA primer/probe sets do not necessarily correlate well with solventdegradation rates.
Proteobacteria (mostly Desulfovibrio sp.). Phylogeneticassociation indicates that only minor shifts in the microbialcommunity structure occurred between the four alternateelectron donor enrichments and the parent consortium.Inconsistent detection of Dehalococcoides spp. in clonelibraries and T-RFLP of enrichment subcultures wasresolved using quantitative polymerase chain reaction (Q-PCR). Q-PCR with primers specific to Dehalococcoides16S rDNA resulted in positive detection of this species inall enrichments. Our results suggest that TCE-dechlorinatingconsortia can be stably maintained on a variety of electrondonors and that quantities of Dehalococcoides cellsdetected with Dehalococcoides specific 16S rDNA primer/probe sets do not necessarily correlate well with solventdegradation rates.