Development of Suspension Polymerized Molecularly Imprinted Beads with Metergoline as Template and Application in a Solid-Phase Extraction Procedure toward Ergot Alkaloids

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• 作者：Pieterjan Lenain ; Jos茅 Diana Di Mavungu ; Peter Dubruel ; Johan Robbens ; Sarah De Saeger
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：December 4, 2012
• 年：2012
• 卷：84
• 期：23
• 页码：10411-10418
• 全文大小：353K
• 年卷期：v.84,no.23(December 4, 2012)
• ISSN：1520-6882

文摘

The first successfully developed molecularly imprinted polymer toward six ergot alkaloids and their respective epimers is described. A new imprinting molecule, metergoline, was used as template analogue in the production of suspension polymerized beads. These spherical particles functioned as selective sorbent in a solid-phase extraction column. The application of this column in the cleanup of barley samples prior to liquid chromatography coupled with tandem mass spectrometry allowed simple and cost-efficient sample preparation. The performance of the imprinted polymer and a non-imprinted control polymer was evaluated. This includes determination of the recovery values and the matrix effect of each of the 12 tested ergot alkaloids as well as a cross-reactivity study with 25 common mycotoxins. The binding isotherms were obtained for metergoline, thus allowing comparison with other (imprinted) sorbents. A comparison between bulk and suspension polymerization is provided to determine the appropriate production technique.