Screening and Identification of Antioxidants in Biological Samples Using High-Performance Liquid Chromatography-Mass Spectrometry and Its Application on Salacca edulis Reinw
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- 作者：Guanghou Shui and Lai Peng Leong
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2005
- 出版时间：February 23, 2005
- 年：2005
- 卷：53
- 期：4
- 页码：880 - 886
- 全文大小：131K
- 年卷期：v.53,no.4(February 23, 2005)
- ISSN：1520-5118
文摘
In this study, a new approach was developed for screening and identifying antioxidants in biologicalsamples. The approach was based on significant decreases of the intensities of ion peaks obtainedfrom high-performance liquid chromatography (HPLC) coupled with mass spectrometry (MS) uponreaction with 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) free radicals. HPLC-MS/MS was further applied to elucidate structures of antioxidant peaks characterized in a spiking test.The new approach could also be used to monitor the reactivity of antioxidants in biological samplewith free radicals. The approach was successfully applied to the identification of antioxidants in salak(Salacca edulis Reinw), a tropical fruit that is reported to be a very good source of natural antioxidants,but it was still not clear which compounds were responsible for its antioxidant property. The antioxidantsin salak were identified to be chlorogenic acid, (-)-epicatechin, and singly linked proanthocyanidinsthat mainly existed as dimers through hexamers of catechin or epicatechin. In salak, chlorogenicacid was identified to be an antioxidant of the slow reaction type as it reacted with free radicals muchmore slowly than either (-)-epicatechin or proanthocyanidins. The new approach was proved to beuseful for the characterization and identification of antioxidants in biological samples as a mass detectorcombined with an HPLC separation system not only serves as an ideal tool to monitor free radicalactive components but also provides their possible chemical structures in a biological sample.Keywords: Salak; antioxidant; HPLC-MS; ABTS free radical; flavan-3-ols; chlorogenic acid