Magnetite Nanoparticles for Stem Cell Labeling with High Efficiency and Long-Term in Vivo Tracking
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- 作者：Noelia GuldrisBárbara Argibay ; Juan Gallo ; Ramón Iglesias-ReyEnrique Carbó-Argibay ; Yury V. Kolen’ko ; Francisco Campos ; Tomás SobrinoLaura M. Salonen ; Manuel Bañobre-López ; José Castillo ; José Rivas
- 刊名：Bioconjugate Chemistry
- 出版年：2017
- 出版时间：February 15, 2017
- 年：2017
- 卷：28
- 期：2
- 页码：362-370
- 全文大小：521K
- ISSN：1520-4812
文摘
Superparamagnetic iron oxide nanoparticles (SPIO-PAA), ultrasmall iron oxide nanoparticles (USPIO-PAA), and glucosamine-modified iron oxide nanoparticles (USPIO-PAA-GlcN) were studied as mesenchymal stem cell (MSCs) labels for cell tracking applications by magnetic resonance imaging (MRI). Pronounced differences were found in the labeling performance of the three samples in terms of cellular dose and labeling efficiency. In combination with polylysine, SPIO-PAA showed nonhomogeneous cell internalization, while for USPIO-PAA no uptake was found. On the contrary, USPIO-PAA-GlcN featured high cellular uptake and biocompatibility, and sensitive detection in both in vitro and in vivo experiments was found by MRI, showing that glucosamine functionalization can be an efficient strategy to increase cell uptake of ultrasmall iron oxide nanoparticles by MSCs.